1. 1. Glucose uptake by isolated rat soleus muscle was stimulated by insulin, anoxia, 2,4-dinitrophenol and salicylate. Anoxia and salicylate both stimulated glucose uptake by increasing the vmax of this process. 2. 2. Anoxia, 2,4-dinitrophenol and salicylate, but not insulin, depleted the ATP content. When muscles were incubated at 21° anoxia stimulated glucose uptake, but did not affect the ATP concentration. 3. 3. ATP (5 mM) added to the incubation medium inhibited glucose uptake under anaerobic conditions, but not aerobically. In the presence of 2,4-dinitrophenol, salicylate or insulin, ATP was without effect. 4. 4. Inhibition of anaerobic glucose uptake was specific to ATP; neither GTP, ITP nor the breakdown products of ATP were effective in this respect. ATP inhibited glucose uptake competitively. This inhibition was not due to chelation of divalent cations. 5. 5. From these experiments it is concluded that the effect of anoxia on sugar transport is not directly mediated via ATP. © 1970.