A simple cell-based high throughput screening (HTS) assay for inhibitors of Salmonella enterica RNA polymerase containing the general stress response regulator RpoS (σ S )

Academic Article


  • RNA polymerase containing the stress response regulator σ S subunit (RpoS) plays a key role in bacterial survival in hostile environments in nature and during infection. Here we devise and validate a simple cell-based high throughput luminescence assay for this holoenzyme suitable for screening large chemical libraries in a robotic platform.
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    Digital Object Identifier (doi)

    Author List

  • Campos-Gomez J; Benitez JA
  • Start Page

  • 1
  • End Page

  • 4
  • Volume

  • 150