Immunosuppressive factor(s) from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT). V. Inhibition of T cell proliferative responses

Academic Article

Abstract

  • The synthetic terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) fails to stimulate development of GAT-specific antibody responses and fails to prime T cells for a subsequent proliferative response, but GAT does stimulate GAT-specific suppressor T cells in nonresponder mice. We have previously shown that extracts from GAT-primed nonresponder T cells contain a suppressor factor that inhibits development of GAT-specific PFC responses by spleen cells from nonresponder mice stimulated with the immunogenic complex, GAT-MBSA. Since GAT-MBSA primes lymph node T cells for development of proliferative responses to GAT in both responder and nonresponder mice, we examined the T cell proliferative response for susceptibility to GAT-primed extracts. The experiments reported in this communication demonstrate that: a) lymphoid cell extracts from GAT-primed nonresponder mice inhibit proliferative responses stimulated by GAT but not MBSA in cultures of GAT-MBSA-primed syngeneic lymph node T cells; b) extracts from GAT-primed nonresponder mice do not inhibit GAT-specific proliferative responses by T cells from (responder x nonresponder)F1 mice; and c) that the active mediator in these extracts binds to GAT, bears no detectable Ig constant region determinants, but does bear determinants encoded by the I-J subregion of the H-2 complex.
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    Author List

  • Araneo BA; Kapp JA
  • Start Page

  • 118
  • End Page

  • 123
  • Volume

  • 125
  • Issue

  • 1