Development of an anti-angiogenic therapeutic model combining scAAV2-delivered siRNAs and noninvasive photoacoustic imaging of tumor vasculature development

Academic Article

Abstract

  • We aimed to develop an anti-angiogenic model for breast cancer by combining (1) siRNA-based therapy delivered by self-complementary adeno-associated virus serotype 2 (scAAV2) vectors to target tumor vasculature, and (2) noninvasive monitoring to tumor response to anti-angiogenesis by photoacoustic (PA) imaging. scAAV2 vector containing 7 surface exposed tyrosine to phenylanine capsid mutations was able to transduce microvascular endothelial cells with high efficiency. siRNAs against UPR (unfolded protein response)-IRE1α, XBP-1, ATF6 significantly inhibited breast cancer-induced angiogenesis in vitro by inhibiting endothelial cell survival. PA imaging showed that knockdown of UPR proteins greatly reduced tumor angiogenesis in vivo in breast cancer models. © 2012 Elsevier Ireland Ltd.
  • Authors

    Published In

  • Cancer Letters  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 17972991
  • Author List

  • Ruan Q; Xi L; Boye SL; Han S; Chen ZJ; Hauswirth WW; Lewin AS; Boulton ME; Law BK; Jiang WG
  • Start Page

  • 120
  • End Page

  • 129
  • Volume

  • 332
  • Issue

  • 1