Claudin-4 induction by E-protein activity in later stages of CD4/8 double-positive thymocytes to increase positive selection efficiency

Academic Article


  • Claudins (Clds) are crucial constituents of tight-junction strands in epithelial cells and have a central role in barrier functions. We show that Cld4 is unexpectedly expressed in normal thymic lymphocytes independently of tight junctions. The Cld4 expression was mostly confined to a portion of the CD4/CD8 double-positive (DP) cells. The proportion of Cld4+ DP cells was markedly increased in MHC-I-/- II-/- mice but decreased in Roraγ-/- mice, and Cld4+ DP cells contained higher levels of the rearranged Tcra transcripts involving the most distal Va and Ja segments than Cld4- DP cells. The Cld4 expression levels were reduced in E47-deficient mice in a gene dose-dependent manner, and ChIP analysis indicated that E2A and HEB were bound to the E-box sites of the putative Cldn4 promoter region. Functionally, Cld4 showed a potent T-cell receptor costimulatory activity by coligation with CD3. The Cld4 was distributed diffusely on the cell surface and associated with CD4/lck independently of CD3 in the resting thymocytes. However, Cld4 was strongly recruited to the immunological synapse on specific T-cell receptor engagement through antigen-presenting cells. In the fetal thymic organ culture, knockdown of Cldn4 resulted in the reduced generation of CD4/CD8 single-positive cells from the DP cells. These results suggest that Cld4 is induced by E-protein activity in the later stages of DP cells to increase the efficiency of positive selection, uncovering a hitherto unrecognized function of a Cld family protein.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 9751781
  • Author List

  • Kawai Y; Hamazaki Y; Fujita H; Fujita A; Sato T; Furuse M; Fujimoto T; Jetten AM; Agata Y; Minato N
  • Start Page

  • 4075
  • End Page

  • 4080
  • Volume

  • 108
  • Issue

  • 10