Differential interleukin-8 response of intestinal epithelial cell line to reactogenic and nonreactogenic candidate vaccine strains of Vibrio cholerae.

Academic Article

Abstract

  • In this study, we analyzed whether attachment of Vibrio cholerae vaccine strains to human intestinal epithelial cells can induce an interleukin-8 (IL-8) response. The IL-8 transcripts were detected by PCR amplification of reverse-transcribed mRNA, and the gene product secretion was measured by an enzyme-linked immunosorbent assay. Infection of monolayers of the undifferentiated HT29-18N2 cell line with reactogenic (JBK70 and 81) and nonreactogenic (CVD103HgR and 638) vaccine strains of V. cholerae resulted in markedly higher IL-8 expression by epithelial cells exposed to reactogenic strains than by cells exposed to the nonreactogenic strains. Additionally, epithelial cells produced IL-8 transcripts following stimulation with cholera vaccine strains in a concentration-dependent manner. These results represent a new insight into the inflammatory component of reactogenicity and could be used as a predictive marker of vaccine reactogenicity prior to human testing.
  • Keywords

  • Bacterial Adhesion, Cholera Vaccines, HT29 Cells, Humans, Interleukin-8, Intestinal Mucosa, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Vibrio cholerae
  • Digital Object Identifier (doi)

    Author List

  • Rodríguez BL; Rojas A; Campos J; Ledon T; Valle E; Toledo W; Fando R
  • Start Page

  • 613
  • End Page

  • 616
  • Volume

  • 69
  • Issue

  • 1