Relationship between beta2-microglobulin and cell-surface alloantigens of the mouse.

Academic Article


  • Molecular relationships between beta2m and other cell surface antigens (H-2, Tla, Ia, and Thy-1) were studied with the double immunofluorescence method. Cells were incubated with an antiserum against one antigen capped, and then tested with an antiserum against a second antigen. Capping of beta2m on thymocytes led to simultaneous capping of H-2 and Tla but not Thy-1 antigens; capping of H-2 and TIa (but not Thy-1) antigens resulted in capping of all beta2m detectable by the immunofluorescence method. Similarly, capping of beta2m on B or T lymphocytes resulted in capping of H-2 and vice versa. Ia antigens on B lymphocytes were not capped after the redistribution of beta2m. We conclude from these data that, in the cell membrane of thymocytes, virtually all the beta2m molecules are associated with H-2 and Tla, but not with Thy-1, and that on the cell surface of T or B lymphocytes, virtually all beta2m is associated with H-2 but not with Ia. We found no evidence of any significant free beta2m on either thymocytes or splenocytes.
  • Published In


  • Animals, B-Lymphocytes, Beta-Globulins, Cell Membrane, Cross Reactions, Female, Fluorescent Antibody Technique, Histocompatibility Antigens, Isoantigens, Male, Mice, Mice, Inbred C57BL, T-Lymphocytes, beta 2-Microglobulin
  • Author List

  • Geib R; Poulik MD; Vitetta ES; Kearney JF; Klein J
  • Start Page

  • 1532
  • End Page

  • 1537
  • Volume

  • 117
  • Issue

  • 5 Pt 1