Expression of immunoglobulin heavy chain at a high level in the absence of a proposed immunoglobulin enhancer element in cis.

Academic Article

Abstract

  • The major intron between the J and C gene segments of the immunoglobulin heavy (H) chain locus contains an enhancer-like sequence, and it has been proposed that this enhancer is necessary to achieve high levels of H chain expression. We have isolated a subclone of the lymphoid pre-B cell line 18-81 that lacks this enhancer but nevertheless produces mu chain at the level characteristic of pre-B cells. Another subclone with a larger deletion does not produce mu chain, but upon fusion with a myeloma that does not produce any immunoglobulin chain, mu chain is expressed by the homolog from the pre-B subclone. The hybridoma lacks the proposed enhancer element in cis; nevertheless it produces as much mu chain as other plasma cell hybridomas. Therefore, this enhancer element is not obligatory for a high level of H chain production.
  • Authors

    Keywords

  • Alleles, Animals, Base Sequence, Cell Line, Clone Cells, DNA, DNA Restriction Enzymes, Flow Cytometry, Genes, Immunoglobulin Heavy Chains, Immunoglobulin mu-Chains, Mice, Nucleic Acid Hybridization, RNA, Messenger
  • Author List

  • Wabl MR; Burrows PD
  • Start Page

  • 2452
  • End Page

  • 2455
  • Volume

  • 81
  • Issue

  • 8