Discordant Analytical Results Caused by Biotin Interference on Diagnostic Immunoassays in a Pediatric Hospital.

Academic Article


  • Recent studies have reported that biotin interferes with certain immunoassays. In this study, we evaluated the analytical interference of biotin on immunoassays that use streptavidin-biotin in our pediatric hospital. We tested the effect of different concentrations of biotin (1.5-200 ng/ml) on TSH, Prolactin, Ferritin, CK-MB, β-hCG, Troponin I, LH, FSH, Cortisol, Anti-HAV antibody (IgG and IgM), assays on Ortho Clinical Diagnostic Vitros 5600 Analyzer. Biotin (up to 200 ng/mL) did not significantly affect Troponin I and HAV assays. Biotin (up to 12.5 ng/ml) resulted in <10% bias in CK-MB, β-hCG, AFP, Cortisol, Ferritin assays and biotin >6.25 ng/mL significantly affected TSH (>20% bias) assay. Prolactin was significantly affected even at low levels (Biotin 1.5 ng/mL). Thus, we recommend educating physicians about biotin interference in common immunoassays and adding an electronic disclaimer.
  • Authors


  • Biotin, Immunoassay, Interference, Vitros, Antioxidants, Automation, Laboratory, Binding, Competitive, Biotin, Blood Chemical Analysis, Child, Child Nutritional Physiological Phenomena, Dietary Supplements, Hospitals, Pediatric, Humans, Immunoassay, Indicators and Reagents, Kinetics, Reproducibility of Results, Streptavidin, Texas
  • Author List

  • Ali M; Rajapakshe D; Cao L; Devaraj S
  • Start Page

  • 638
  • End Page

  • 640
  • Volume

  • 47
  • Issue

  • 5