Methods for assessing mitochondrial quality control mechanisms and cellular consequences in cell culture.

Academic Article

Abstract

  • Mitochondrial quality is under surveillance by autophagy, the cell recycling process which degrades and removes damaged mitochondria. Inadequate autophagy results in deterioration in mitochondrial quality, bioenergetic dysfunction, and metabolic stress. Here we describe in an integrated work-flow to assess parameters of mitochondrial morphology, function, mtDNA and protein damage, metabolism and autophagy regulation to provide the framework for a practical assessment of mitochondrial quality. This protocol has been tested with cell cultures, is highly reproducible, and is adaptable to studies when cell numbers are limited, and thus will be of interest to researchers studying diverse physiological and pathological phenomena in which decreased mitochondrial quality is a contributory factor.
  • Published In

  • Redox Biology  Journal
  • Keywords

  • Autophagy, Beta cells, Bioenergetics, Metabolomics, Mitochondrial fragmentation, Mitophagy, Neuron, Animals, Autophagy, Brain, Cell Culture Techniques, DNA, Mitochondrial, Energy Metabolism, Humans, Mice, Mitochondria, Mitochondrial Degradation, Neurons, Quality Control, Rats
  • Digital Object Identifier (doi)

    Pubmed Id

  • 26355348
  • Author List

  • Redmann M; Benavides GA; Wani WY; Berryhill TF; Ouyang X; Johnson MS; Ravi S; Mitra K; Barnes S; Darley-Usmar VM
  • Start Page

  • 59
  • End Page

  • 69
  • Volume

  • 17