Evaluation of a real-time PCR assay for detection of Mycoplasma genitalium and macrolide resistance-mediating mutations from clinical specimens.

Academic Article

Abstract

  • Mycoplasma genitalium (MG) is a sexually transmitted pathogen for which there is no FDA-approved diagnostic test available in the United States. A modified real-time polymerase chain reaction assay for detecting MG and simultaneously identifying macrolide resistance mutations from clinical specimens was evaluated and proved to be sensitive and accurate for diagnostic purposes.
  • Keywords

  • Macrolide, Mycoplasma genitalium, Real-time PCR, Resistance, Anti-Bacterial Agents, DNA, Bacterial, Drug Resistance, Bacterial, Humans, Macrolides, Mutation, Mycoplasma Infections, Mycoplasma genitalium, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Sensitivity and Specificity
  • Digital Object Identifier (doi)

    Author List

  • Xiao L; Waites KB; Wang H; Van Der Pol B; Ratliff AE; Geisler WM
  • Start Page

  • 123
  • End Page

  • 125
  • Volume

  • 91
  • Issue

  • 2