Baculoviral p35 inhibits oxidant-induced activation of mitochondrial apoptotic pathway

Academic Article

Abstract

  • In this study we report that the baculovirus p35 anti-apoptotic protein prevents cell death by quenching free radicals at a very upstream step in the apoptotic pathway. Mitochondria of activated rat peritoneal macrophages as well as Spodoptera frugiperda (Sf9) insect cells, following treatment with oxidants, H2O2/UVB irradiation, release cytochrome c followed by activation of caspase-3. Transfection of macrophages/Sf9 cells with a construct carrying the p35 gene under the CMV/HSP promoters resulted in p35 expression and consequent arrest of oxidative stress-induced apoptosis. p35 expression also inhibited cytochrome c release from the mitochondria of oxidant-exposed cells and blocked caspase-3 activation. © 2003 Elsevier Inc. All rights reserved.
  • Digital Object Identifier (doi)

    Author List

  • Sahdev S; Taneja TK; Mohan M; Sah NK; Khar AK; Hasnain SE; Athar M
  • Start Page

  • 483
  • End Page

  • 490
  • Volume

  • 307
  • Issue

  • 3