Crystallization and preliminary cryogenic x-ray diffraction analyses of protein L-isoaspartyl O-methyltransferase from human fetal brain

Academic Article

Abstract

  • Recombinant human fetal brain protein L-isoaspartyl O- methyltransferase, EC 2.1.1.77, was crystallized in PEG 8000 with adenosine homocysteine by a macroseeding technique. The space group was P21 with a = 47.4 Å, b = 53.9 Å, c = 48.7 Å and β = 116.4°for cryofrozen crystals at 90 K. The crystals diffracted to 2.1 Å and have one molecule per asymmetric unit.
  • Published In

  • Proteins  Journal
  • Author List

  • Smith CD; Barchue J; Mentel C; DeLucas L; Shirasawa T; Chattopadhyay D
  • Start Page

  • 457
  • End Page

  • 460
  • Volume

  • 28
  • Issue

  • 3