© 2014, Sphinx Knowledge House. All rights reserved. An attempt was made to investigate the effect of Lufenuron in liver tissue of mice, Musculus species. The sublethal dose of Lufenuron (0.1520 mg/kg) administered to mice. In the present study the level of Lipid Peroxidation (LPO), Glutathione (GSH), Glutathione Peroxidase (GPx), Catalase (CAT) and Superoxide Dismutase (SOD) were observed. Also as a liver function marker serum, Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Alkaline phosphatase was estimated. The present study suggests that the level of lipid peroxidation was increased and glutathione, catalase and superoxide dismutase were significantly decreased in the liver tissue of Lufenuron exposed mice. The liver enzymes ALT, AST and ALP were also increased in the treated animals. The present study concludes that the Lufenuron damages the liver tissue of mice.