Nα-acetyltransferase deficiency alters protein synthesis in Saccharomyces cerevisiae

Academic Article


  • Acetylation is the most frequently occurring chemical modification of the α-NH2 group of eukaryotic proteins and is catalyzed by a Nα-acetyltransferase. Two-dimensional gel electrophoresis was used to compare the soluble proteins synthesized in wild type and a mutant (aaal) yeast cells lacking Nα-acetyltransferase. Among 855 soluble proteins identified in wild type and mutant, ~20% of the proteins in the mutant either disappeared or were shifted to higher pI without a change of molecular mass, and 27 proteins were observed only in the mutant. In addition, the synthesis of another 12% of the proteins in the mutant was either diminished or enhanced, suggesting that the acetylation of certain regulatory proteins may affect their expression. This is the first demonstration of the broad-based functional role of Nα-acetylation in eukaryotic protein synthesis. Acetyltransferase; Acetylation; (Saccharomyces cerevisiae). © 1989.
  • Authors

    Published In

  • FEBS Letters  Journal
  • Digital Object Identifier (doi)

    Author List

  • Lee FJS; Lin LW; Smith JA
  • Start Page

  • 139
  • End Page

  • 142
  • Volume

  • 256
  • Issue

  • 1-2