Human tumor cells synthesize an endothelial cell growth factor that is structurally related to basic fibroblast growth factor

Academic Article

Abstract

  • A human hepatoma cell line synthesizes, as evidenced by metabolic labeling, an endothelial cell mitogen that is found to be mostly cell associated. The hepatoma-derived growth factor (HDGF) has been purified to homogeneity by a combination of Bio-Rex 70, heparin-Sepharose, and reverse-phase chromatography; it is a cationic polypeptide with a molecular weight of about 18,500-19,000. HDGF is structurally related to basic fibroblast growth factor (FGF). Immunological analysis demonstrates that antiserum prepared against a synthetic peptide corresponding to the amino-terminal sequence of basic FGF cross-reacts with HDGF when analyzed by electrophoretic blotting and by immunoprecipitation. Sequence analysis of tryptic fragments demonstrates that HDGF contains sequences that are homologous to both amino-terminal and carboxyl-terminal sequences of basic FGF.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Klagsbrun M; Sasse J; Sullivan R; Smith JA
  • Start Page

  • 2448
  • End Page

  • 2452
  • Volume

  • 83
  • Issue

  • 8