N(α) acetylation is required for normal growth and mating of Saccharomyces cerevisiae

Academic Article


  • Acetylation is the most frequently occurring chemical modification of the α-NH2 group of eucaryotic proteins and is catalyzed by N(α)-acetyltransferase. The yeast enzyme is encoded by the AAA1 (amino-terminal α-amino acetyltransferase) gene. A null mutation (aaa1-1) created by gene replacement, while not lethal, slows cell growth and results in heterogeneous colony morphology. In comparison with wild-type cells, aaa1-1/aaa1-1 diploids cannot enter stationary phase, are sporulation defective, and are sensitive to heat shock. In addition, the aaa1-1 mutation specifically reduces mating functions of MATa cells. These results indicate that N(α) acetylation plays a crucial role in yeast cell growth and mating.
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    Digital Object Identifier (doi)

    Author List

  • Lee FJS; Lin LW; Smith JA
  • Start Page

  • 5795
  • End Page

  • 5802
  • Volume

  • 171
  • Issue

  • 11