Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis

Academic Article

Abstract

  • Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in < 8 min by high-performance capillary electrophoresis with polyacrylamide gels. In addition, fast analysis of a crude 70-mer oligodeoxynucleotide and a slab gel-purified 99-mer oligodeoxynucleotide was accomplished, demonstrating the ability of high-performance capillary electrophoresis to characterize rapidly synthesized oligonucleotides. Besides analytical separations, 800 ng of a primer (20-mer) was isolated in < 20 min. The purified species was collected in water and subsequently used as a probe in a standard dot-blot analysis. The use of high-performance capillary electrophoresis for the analysis and purification of a variety of biopolymers is simple, rapid, and has the potential for automation.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Cohen AS; Najarian DR; Paulus A; Guttman A; Smith JA; Karger BL
  • Start Page

  • 9660
  • End Page

  • 9663
  • Volume

  • 85
  • Issue

  • 24