The t(10;14)(q24;q11) of T-cell acute lymphoblastic leukemia juxtaposes the δ T-cell receptor with TCL3, a conserved and activated locus at 10q24

Academic Article

Abstract

  • We cloned the t(10;14) recurrent translocation from CD3-negative T-cell acute lymphoblastic leukemia cells. The breakpoint at 14q11 involved an intermediate rearrangement of the δ T-cell receptor locus, suggesting that the translocation arose at the time of antigen receptor assemblage. Translocation introduced chromosome segment 10q24 as proven by hybridization of a breakpoint-derived probe to flow-sorted chromosomes and metaphase chromosomes. Two t(10;14) breakpoints were clustered within a 600-base-pair region of 10q24 but no heptamer-spacer-nonamer motifs resembling T-cell receptor/immunoglobulin rearrangement signals were noted at the breakpoint. A locus distinct from terminal deoxynucleotidyltransferase was found at 10q24. Evolutionarily conserved regions surrounding the 10q24 breakpoint were examined for transcriptional activity. A region telomeric to the 10q24 breakpoint, expected to translocate to the der(14) chromosome, recognized an abundant 2.9-kilobase RNA in a t(10;14) T-cell leukemia. This locus was not active in a variety of other normal and neoplastic T cells, arguing that it was deregulated by the introduction of the T-cell receptor. This locus is a candidate for a putative protooncogene, TCL3, involved in T-cell neoplasia.
  • Digital Object Identifier (doi)

    Author List

  • Zutter M; Hockett RD; Roberts CWM; McGuire EA; Bloomstone J; Morton CC; Deaven LL; Crist WM; Carroll AJ; Korsmeyer SJ
  • Start Page

  • 3161
  • End Page

  • 3165
  • Volume

  • 87
  • Issue

  • 8