Mapping the 3D orientation of piconewton integrin traction forces

Academic Article

Abstract

  • Mechanical forces are integral to many biological processes; however, current techniques cannot map the magnitude and direction of piconewton molecular forces. Here, we describe molecular force microscopy, leveraging molecular tension probes and fluorescence polarization microscopy to measure the magnitude and 3D orientation of cellular forces. We mapped the orientation of integrin-based traction forces in mouse fibroblasts and human platelets, revealing alignment between the organization of force-bearing structures and their force orientations.
  • Published In

  • Nature methods  Journal
  • Digital Object Identifier (doi)

    Author List

  • Brockman JM; Blanchard AT; Pui-Yan V; Derricotte WD; Zhang Y; Fay ME; Lam WA; Evangelista FA; Mattheyses AL; Salaita K
  • Start Page

  • 115
  • End Page

  • 118
  • Volume

  • 15
  • Issue

  • 2