Direct observation of membrane insertion by enveloped virus matrix proteins by phosphate displacement.

Academic Article

Abstract

  • Enveloped virus release is driven by poorly understood proteins that are functional analogs of the coat protein assemblies that mediate intracellular vesicle trafficking. We used differential electron density mapping to detect membrane integration by membrane-bending proteins from five virus families. This demonstrates that virus matrix proteins replace an unexpectedly large portion of the lipid content of the inner membrane face, a generalized feature likely to play a role in reshaping cellular membranes.
  • Authors

    Published In

  • PLoS ONE  Journal
  • Keywords

  • Animals, Cell Line, Cell Membrane, Cryoelectron Microscopy, Phosphates, Protein Binding, Viral Matrix Proteins
  • Digital Object Identifier (doi)

    Author List

  • Neuman BW; Kiss G; Al-Mulla HMN; Dokland T; Buchmeier MJ; Weikl T; Schley D
  • Start Page

  • e57916
  • Volume

  • 8
  • Issue

  • 2