A modified slurry beveler for HRP-filled intracellular micropipettes

Academic Article

Abstract

  • A modified spinning dish type beveler is described which quickly and reliably bevels fine micropipettes. The beveler is easy and inexpensive to construct, and requires little operator skill. Scanning electron micrographs demonstrate that a true bevel is achieved by this method. The design of an impedance measuring device that uses low current at low frequency through the electrode to monitor beveling is shown. Techniques for filling and cleaning the microelectrodes are also discussed in conjunction with coating the electrode tip with a lipophilic substance which facilitates intracellular penetration and stability.
  • Author List

  • Amthor FR
  • Start Page

  • 79
  • End Page

  • 86
  • Volume

  • 11
  • Issue

  • 2