Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5.

Academic Article

Abstract

  • Heme's spin-multiplicity is key in determining the enzymatic function of cytochrome P450 (cytP450). The origin of the low-spin state in ferric P450 is still under debate. Here, we report the first experimental demonstration of P450's membrane interaction altering its spin equilibrium which is accompanied by a stronger affinity for cytochrome b5. These results highlight the importance of lipid membrane for the function of P450.
  • Published In

    Keywords

  • Cytochrome P-450 Enzyme System, Cytochromes b5, Models, Molecular
  • Digital Object Identifier (doi)

    Author List

  • Ravula T; Barnaba C; Mahajan M; Anantharamaiah GM; Im S-C; Waskell L; Ramamoorthy A
  • Start Page

  • 12798
  • End Page

  • 12801
  • Volume

  • 53
  • Issue

  • 95