Staining of retinal neurons in the isolated eyecup by extracellular horseradish peroxidase injection.

Academic Article


  • A reliable method has been developed for the staining of mammalian retinal neurons in the isolated eyecup. Small injections of horseradish peroxidase are made at a number of places on the retinal surface while the hemisected eyecup preparation floats in Eagle's medium. After 30 min, the retinas are removed, fixed, and reacted with diaminobenzidine. Golgi-like staining of fine dendrites, spines and axons of ganglion cells is achieved. The method also stains amacrine, horizontal and bipolar cells.
  • Published In

  • Vision Research  Journal
  • Keywords

  • Animals, Cats, Cytological Techniques, Ferrets, Horseradish Peroxidase, Neurons, Rabbits, Retina, Retinal Ganglion Cells, Staining and Labeling
  • Author List

  • Amthor FR; Jackson CA
  • Start Page

  • 269
  • End Page

  • 274
  • Volume

  • 26
  • Issue

  • 2