Staining of retinal neurons in the isolated eyecup by extracellular horseradish peroxidase injection

Academic Article

Abstract

  • A reliable method has been developed for the staining of mammalian retinal neurons in the isolated eyecup. Small injections of horseradish peroxidase are made at a number of places on the retinal surface while the hemisected eyecup preparation floats in Eagle's medium. After 30 min, the retinas are removed, fixed, and reacted with diaminobenzidine. Golgi-like staining of fine dendrites, spines and axons of ganglion cells is achieved. The method also stains amacrine, horizontal and bipolar cells. © 1986.
  • Digital Object Identifier (doi)

    Author List

  • Amthor FR; Jackson CA
  • Start Page

  • 269
  • End Page

  • 274
  • Volume

  • 26
  • Issue

  • 2