Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs

Academic Article

Abstract

  • Numerous recent studies have suggested that the predicted cytosolic domains of G protein-coupled receptors represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G proteins. This Chapter describes two in vitro methods for ascertaining interactions between G protein-coupled receptors and various binding partners: gel overlay strategies and GST-fusion protein pull-downs. © Springer Science+Business Media, LLC 2011.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Brady AE; Chen Y; Limbird LE; Wang Q
  • Start Page

  • 347
  • End Page

  • 355
  • Volume

  • 746