Genome-based expression profiles as a single standardized microarray platform for the diagnosis of experimental interstitial cystitis: An array of 75 genes model

Academic Article

Abstract

  • Introduction and hypothesis: To investigate the molecular signature underlying experimental interstitial cystitis (IC) using cDNA microarray. Methods: Microarray gene expression profiles are studied inbladder epithelium of C57BL/6 mice with ovalbumin or substance P-induced experimental IC versus Escherichia coli lipopolysaccharide-induced bacterial cystitis. Results: Main findings are summarized as follows: firstly, a '75-gene' model was discovered to contain high expressions of bladder epithelium which feature in experimental IC. Secondly, glucose, lipid, nucleotide, xenobiotics, and amino acid metabolisms are involved in. Thirdly, T-cell-mediated immune and inflammatory responses are observed. Fourthly, Wnt, Tgf-beta, Mapk, and insulin growth factor receptor signaling pathways are also involved in. In addition, experimental IC leads to Ephrin- and Semaphorin-mediated axon guidance promoting parasympathetic inflammatory reflexes. Conclusions: Further characterization of human IC-induced gene expression profiles would enable the use of genome-based expression profiling for the therapeutic targets and diagnosis of IC. © The International Urogynecological Association 2009.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Tseng LH; Chen I; Chen MY; Lee CL; Lo TS; Lloyd LK
  • Start Page

  • 509
  • End Page

  • 513
  • Volume

  • 20
  • Issue

  • 5