Genome-based expression profiles as a single standardized microarray platform for the diagnosis of experimental interstitial cystitis: an array of 75 genes model.

Academic Article

Abstract

  • INTRODUCTION AND HYPOTHESIS: To investigate the molecular signature underlying experimental interstitial cystitis (IC) using cDNA microarray. METHODS: Microarray gene expression profiles are studied in bladder epithelium of C57BL/6 mice with ovalbumin or substance P-induced experimental IC versus Escherichia coli lipopolysaccharide-induced bacterial cystitis. RESULTS: Main findings are summarized as follows: firstly, a "75-gene" model was discovered to contain high expressions of bladder epithelium which feature in experimental IC. Secondly, glucose, lipid, nucleotide, xenobiotics, and amino acid metabolisms are involved in. Thirdly, T-cell-mediated immune and inflammatory responses are observed. Fourthly, Wnt, Tgf-beta, Mapk, and insulin growth factor receptor signaling pathways are also involved in. In addition, experimental IC leads to Ephrin- and Semaphorin-mediated axon guidance promoting parasympathetic inflammatory reflexes. CONCLUSIONS: Further characterization of human IC-induced gene expression profiles would enable the use of genome-based expression profiling for the therapeutic targets and diagnosis of IC.
  • Authors

    Keywords

  • Animals, Cluster Analysis, Cystitis, Interstitial, Ephrins, Escherichia coli, Gene Expression Profiling, Lipopolysaccharides, Lymphocyte Activation, MAP Kinase Signaling System, Mice, Mice, Inbred C57BL, Nitric Oxide Synthase Type III, Oligonucleotide Array Sequence Analysis, Ovalbumin, PAX2 Transcription Factor, Semaphorins, Substance P, Transcriptome, Up-Regulation, Wnt Signaling Pathway
  • Digital Object Identifier (doi)

    Authorlist

  • Tseng L-H; Chen I; Chen M-Y; Lee C-L; Lo T-S; Lloyd LK
  • Start Page

  • 509
  • End Page

  • 513
  • Volume

  • 20
  • Issue

  • 5