Freshly isolated Thy-1+ dendritic epidermal cells express T cell receptor γδ-CD3

Academic Article

Abstract

  • Within murine epidermis exists a population of Thy-1+ DEC which express membrane Thy-1 antigen, but lack CD8 or CD4 antigen. We examined freshly obtained non-cultured Thy-1+ DEC both by immunofluorescence and by biochemical techniques to identify the protein products of the T cell receptor (TCR) and the associated CD3 complex on these cells. Virtually all of the Thy-1+ DEC are brightly positive in CD3 expression with immunofluorescence using the monoclonal antibody 145-2C11. By immunoprecipitation, using this same antibody and polyclonal anti-TCR-γ antibody, the only TCR heterodimer detected on the freshly isolated Thy-1+ DEC is the γδ heterodimer. These findings suggest that in the phenotype and TCR expression, Thy-1+ DEC are analogous to CD8-, CD4- early fetal thymocytes. © 1990.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Shimada S; Caughman SW; Bluestone JA; Cron RQ; Owen FL; Smith JA; Katz SI
  • Start Page

  • 459
  • End Page

  • 464
  • Volume

  • 1
  • Issue

  • 6