Chemical and enzymatic synthesis of 4'-thio-beta-D-arabinofuranosylcytosine monophosphate and triphosphate.

Academic Article

Abstract

  • N4-Acetyl-1-(2, 3-di-O-acetyl-4-thio-beta-D-arabinofuranosyl) cytosine (2) was synthesized in three steps from 1-(4-thio-beta-D-arabinofuranosyl) cytosine (1). The reaction of this partially blocked 4'-thio-ara-C derivative 2 with 2-chloro-4H-1,3,2-benzodioxaphosphorin-4-one gave the 5-phosphitylate derivative 3, which on reaction with pyrophosphate gave the 5'-nucleosidylcyclotriphosphite 4. Product 4 was then oxidized with iodine/pyridine/water and deblocked with concentrated ammonium hydroxide to provide the desired 4'-thio-ara-C-5'-triphosphate 5. This triphosphate 5 was converted to 4'-thio-ara-C -5'-monophosphate 6 by treatment with snake venom phosphodiesterase I. The details of the synthesis, purification, and characterization of both nucleotides are described.
  • Published In

    Keywords

  • Ammonium Hydroxide, Arabinofuranosylcytosine Triphosphate, Arabinonucleotides, Chemistry, Pharmaceutical, Chromatography, Ion Exchange, Cytidine Monophosphate, Cytidine Triphosphate, Diphosphates, Drug Design, Hydroxides, Models, Chemical, Molecular Structure, Phosphodiesterase I, Polyphosphates, Water
  • Authorlist

  • Fowler AS; Tiwari KN; Campbell SR; Secrist JA
  • Start Page

  • 533
  • End Page

  • 537
  • Volume

  • 24
  • Issue

  • 5-7