Nano-TiO₂ particles impair adhesion of airway epithelial cells to fibronectin.

Academic Article


  • Titanium dioxide engineered nanoparticles (nano-TiO(2)) are widely used in the manufacturing of a number of products. Due to their size (<100 nm), when inhaled they may be deposited in the distal lung regions and damage Clara cells. We investigated the mechanisms by which short-term (1-h) incubation of human airway Clara-like (H441) cells to nano-TiO(2) (6 nm in diameter) alters the ability of H441 cells to adhere to extracellular matrix. Our results show that 1h post-incubation, there was a 3-fold increase of extracellular H(2)O(2), increased intracellular oxidative stress as demonstrated by 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) oxidation, and a 5-fold increase of phosphor-ERK1/2 as measured by Western blotting. These changes were accompanied by a 25% decrease of H441 adherence to fibronectin (p<0.05 compared to vehicle incubated H441 cells). Pretreatment with the ERK1/2 inhibitor U0126 for 3h, partially prevented this effect. In conclusion, short-term exposure of H441 cells to nano-TiO(2) appears to reduce adherence to fibronectin due to oxidative stress and activation of ERK1/2.
  • Keywords

  • Adenocarcinoma, Analysis of Variance, Cell Adhesion, Cell Death, Cell Line, Tumor, Dose-Response Relationship, Drug, Epithelial Cells, Humans, Hydrogen Peroxide, Metal Nanoparticles, Microscopy, Electron, Scanning, Mitogen-Activated Protein Kinase 1, Oxidative Stress, Reactive Oxygen Species, Spectrophotometry, Infrared, Time Factors, Titanium, Transcription Factors
  • Digital Object Identifier (doi)

    Author List

  • Zarogiannis SG; Filippidis AS; Fernandez S; Jurkuvenaite A; Ambalavanan N; Stanishevsky A; Vohra YK; Matalon S
  • Start Page

  • 454
  • End Page

  • 460
  • Volume

  • 185
  • Issue

  • 2