Genomic organization of mouse Fc(γ) receptor genes

Academic Article

Abstract

  • We have isolated and characterized the gene coding for the mouse Fc receptor that is termed Fc(γ)RIIa. The gene contains five exons and spans approximately 9 kilobases. Unlike most members of the immunoglobulin gene superfamily, this gene utilizes multiple exons to encode its leader peptide. The first exon encodes the hydrophobic region of the signal sequence; the second exon, which contains only 21 base pairs, encodes a segment of the signal peptidase recognition site; and the beginning of the third exon encodes the predicted site of peptidase cleavage. The third and fourth exons each code for immunoglobulin-like extracellular domains. The fifth exon encodes the hydrophobic transmembrane domain and the cytoplasmic tail. Partial characterization of the Fc(γ)RIIb gene indicates that it also contains multiple leader exons, including a 21-base-pair exon and two exons coding for homologous immunoglobulin-like extracellular domains. However, the Fc(γ)RIIb gene uses four exons to encode its intracytoplasmic region. Analysis using contour-clamped homogeneous electric field (CHEF) gels indicates that the Fc(γ)RIIa and Fc(γ)RIIb genes are linked within 160 kilobases on mouse chromosome 1.
  • Digital Object Identifier (doi)

    Author List

  • Kulczycki A; Webber J; Soares HA; Onken MD; Thompson JA; Chaplin DD; Loh DY; Tillinghast JP
  • Start Page

  • 2856
  • End Page

  • 2860
  • Volume

  • 87
  • Issue

  • 7