Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node.

Academic Article

Abstract

  • Lymphocyte rolling velocity is determined largely by interactions between leukocyte alpha(4)-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. With the use of real-time intravital confocal microscopy, we found that rolling velocities of T lymphocytes in the murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in the murine spleen, rolling velocities of T lymphocytes are not influenced by the loss of L-selectin and CD49d. With the use of FITC-dextran and TIE2-GFP mice, we further defined the microvascular compartments of the spleen and showed that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs and suggest that the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ.
  • Keywords

  • Animals, Cell Adhesion, Cell Adhesion Molecules, Cell Separation, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Immunoglobulins, L-Selectin, Lymph Nodes, Mesentery, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Confocal, Microspheres, Mucoproteins, Pertussis Toxin, Regional Blood Flow, Rheology, Spleen, T-Lymphocytes
  • Digital Object Identifier (doi)

    Author List

  • Grayson MH; Hotchkiss RS; Karl IE; Holtzman MJ; Chaplin DD
  • Start Page

  • H2213
  • End Page

  • H2226
  • Volume

  • 284
  • Issue

  • 6