This study was undertaken to determine if there was evidence of impaired gluconeogenesis during shock and to test the effectiveness of steroids on gluconeogenesis during such conditions. Hemorrhagic shock in previously adrenalectomized rats was produced by bleeding the animals to a mean arterial pressure of 40 mm Hg, which was maintained for 2 hr. Liver and kidney slices from control animals and from animals in shock were prepared and incubated under aerobic conditions for 3 hr at 37°C in Krebs-Henseliet bicarbonate buffer containing 10 mM alanine or glutamine in the presence or absence of steroids. Following incubation, glucose and urea production in the medium was measured. Hydrocortisone addition at 10-7M increased the quality of glucose and urea appearing in the medium by 16% in the presence of control slices as well as in the presence of slices from animals in shock. The addition of 10-4M hydrocortisone inhibited gluconeogenesis by 30%, whereas with 10-2M hydrocortisone there was complete inhibition of gluconeogenesis with both groups of slices. Similar results were obtained using dexamethasone or hydrocortisone 21-sodium succinate. Thus, basal gluconeogenic capability was unaltered during shock in an adrenalectomized animal and steroids were as effective in stimulating or inhibiting gluconeogenesis during hemorrhagic shock as they were under control conditions. © 1978.