Subclavian arteries of rats were cannulated and the animals were bled rapidly to a pressure of 40 mm Hg and maintained at that level for 2 hr. Glucose uptake by isolated soleus muscles from normal rats and rats which were subjected to severe hemorrhagic shock (“shock muscles”) was the same although ATP levels were significantly lowered in muscles of the latter rats. Whereas shock muscles did not show increased glucose uptake, these muscles still responded to the in vitro effect of anoxia by showing increased glucose uptake, further depletion of ATP and increased lactic acid production. The present experiments indicate that: (a) during severe hemorrhagic shock, there is no cause-effect relationship between glucose uptake and ATP depletion and (b) whatever the mechanism of action of anoxia in promoting glucose uptake in vitro might be, it is quite different from that of in vivo. © 1973, SAGE Publications. All rights reserved.