Glycolate and 2-phosphoglycolate content of tissues measured by ion chromatography coupled to mass spectrometry

Academic Article

Abstract

  • Glycolate and 2-phosphoglycolate (PG) are 2-carbon monocarboxylic acids with ill-defined metabolic roles. Their concentrations have not yet been described in tissues apart from body fluids and erythrocytes. We describe the use of ion chromatography coupled with mass spectrometry (IC-MS) to quantify levels of glycolate and PG in tissue. Sample preparation and analysis can be performed within an hour. Low concentrations of glycolate (12-48 nmol/g) and PG (4-17 nmol/g) were detected in all tissues. The availability of this IC-MS assay will facilitate investigations of the origin, function, and metabolism of glycolate and PG in tissues. © 2011 Elsevier Inc. All rights reserved.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 11421229
  • Author List

  • Knight J; Hinsdale M; Holmes R
  • Start Page

  • 121
  • End Page

  • 124
  • Volume

  • 421
  • Issue

  • 1