Rapid inactivation of bacteriophage T7 by ascorbic acid is repairable.

Academic Article

Abstract

  • Treatment of bacteriophage T7 with ascorbic acid resulted in the rapid accumulation of single-strand breaks in the DNA with double-strand breaks appearing only after incubation times of 20 min or longer. The single-strand breaks were responsible for a rapid inactivation of the phage as assayed by immediate plating of the phage-bacteria mixture on nutrient agar. Incubation of the phage-bacteria mixture in liquid medium prior to plating allowed a host cell reactivation process to repair the nicks and reactivate the phage. Non-reversible inactivation of the phage was a slower process which could be correlated with the appearance of double-strand breaks in the phage DNA. Host cell reactivation of the phage was also manifested in the phenomena of delayed lysis and delayed appearance of the concatemeric DNA replication intermediate.
  • Authors

    Published In

    Keywords

  • Ascorbic Acid, Coliphages, DNA Repair, DNA Replication, DNA, Single-Stranded, DNA, Viral, Escherichia coli, Kinetics, Species Specificity, Virus Replication
  • Pubmed Id

  • 3803571
  • Authorlist

  • Richter HE; Loewen PC
  • Start Page

  • 25
  • End Page

  • 30
  • Volume

  • 697
  • Issue

  • 1