Preclinical evaluation of the novel monoclonal antibody H6-11 for prostate cancer imaging.

Academic Article

Abstract

  • The biological properties of the novel monoclonal antibody (mAb) H6-11 and its potential utility for oncological imaging studies were evaluated using in vitro and in vivo assays. Immunoreactivity of H6-11 to the human prostate cancer PC-3 cell line and solid tumor xenografts was initially demonstrated using immunofluorescence staining; the specificity of H6-11 for prostate cancer was further evaluated using a commercial array of human prostate cancer and normal tissue samples (n=49) in which H6-11 detected 95% of prostate adenocarcinomas. The Kd value of 61.7±30 nM was determined using 125I-labeled H6-11. Glycosylation analysis suggested the antigenic epitope of the glycan is an O-linked β-N-acetylglucoside (O-GlcNAc) group. Imaging studies of PC-3 tumor-bearing mice were performed using both optical imaging with NIR fluorescent dye-labeled H6-11 and microPET imaging with 89Zr-labeled H6-11. These in vivo studies revealed that the labeled probes accumulated in PC-3 tumors 48-72 h postinjection, although significant retention in liver was also observed. By 120 h postinjection, the tumors were still evident, although the liver showed significant clearance. These studies suggest that the mAb H6-11 may be a useful tool to detect prostate cancer in vitro and in vivo.
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    Published In

    Keywords

  • Animals, Antibodies, Monoclonal, Antigens, Tumor-Associated, Carbohydrate, Cell Line, Tumor, Diagnostic Imaging, Female, Fluorescent Antibody Technique, Glycosylation, Humans, Male, Mice, Mice, Inbred BALB C, Positron-Emission Tomography, Prostatic Neoplasms
  • Digital Object Identifier (doi)

    Authorlist

  • Jin H; Xu M; Padakanti PK; Liu Y; Lapi S; Tu Z
  • Start Page

  • 3655
  • End Page

  • 3664
  • Volume

  • 10
  • Issue

  • 10