Innate immune responses in Swine resuscitated from severe traumatic hemorrhagic shock with hemoglobin-based oxygen carrier-201.

Academic Article

Abstract

  • Hemoglobin-based oxygen carrier-201 transports oxygen and improves survival in swine with hemorrhagic shock, but has potential to be immune activating. Herein, we evaluated HBOC-201's immune effects in swine with more severe hemorrhagic shock due to soft tissue injury and 55% blood volume catheter withdrawal over 15 minutes followed by fluid resuscitation at 20 minutes with HBOC-201, Hextend, or no treatment (NON) before hospital arrival. Survival rates were similar with HBOC-201 and Hextend (p > 0.05), but were higher than in (p = 0.007). There were no significant group differences in blood cell count, percentages of leukocyte sub-populations and immunophenotype (CD4:CD8 ratio), adhesion markers expression (neutrophil CD11b; monocyte or neutrophil CD49d) and apoptosis. There was a trend to higher plasma IL-10 in HBOC-201 and groups vs. Hextend. We conclude that in swine with severe controlled HS and soft tissue injury, immune responses are similar with resuscitation with HBOC-201 and Hextend.
  • Authors

    Keywords

  • Animals, Apoptosis, Blood Pressure, Blood Substitutes, Cytokines, Disease Models, Animal, Drug Evaluation, Preclinical, Emergency Medical Services, Fluid Therapy, Hemoglobins, Hydroxyethyl Starch Derivatives, Immunity, Innate, Resuscitation, Shock, Hemorrhagic, Swine, Swine, Miniature, T-Lymphocytes
  • Digital Object Identifier (doi)

    Author List

  • Hall C; Malkevich N; Handrigan M; Vandermolen C; Aranaud F; Hong J; Dong F; Rice J; Philbin N; Ahlers S
  • Start Page

  • 259
  • End Page

  • 274
  • Volume

  • 35
  • Issue

  • 3