Demonstration of inositol 1,3,4,5-tetrakisphosphate receptor binding

Academic Article

Abstract

  • Inositol 1,3,4,5-tetrakisphosphate (InsP4) is produced rapidly upon stimulation of the phosphoinositide system and may serve as a second messenger in hormone and neurotransmitter action. In this report we demonstrate specific binding sites for [3H]InsP4 in rat tissue membranes. In cerebellar membranes, [3H]InsP4 binding sites are displaced both by InsP4 and inositol 1,4,5-trisphosphate (InsP3) with similar potency (IC50 {reversed tilde equals} 300 nM) whereas several other inositol phosphates are much weaker. We have distinguished the InsP4 binding site from the InsP3 receptor binding site by differences in brain regional and tissue distribution, affinity for InsP4 and InsP3, and sensitivity to calcium. © 1987 Academic Press, Inc.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Theibert AB; Supattapone S; Worley PF; Baraban JM; Meek JL; Snyder SH
  • Start Page

  • 1283
  • End Page

  • 1289
  • Volume

  • 148
  • Issue

  • 3