Rhamnose biosynthesis in mycoplasmas requires precursor glycans larger than monosaccharide

Academic Article

Abstract

  • Despite the apparent absence of genes coding for the known pathways for biosynthesis, the monosaccharide rhamnose was detected in the d configuration in Mycoplasma pneumoniae and Mycoplasma pulmonis, and in both the d and l configurations in Mycoplasma arthritidis. Surprisingly, the monosaccharide glucose was not a precursor for rhamnose biosynthesis and was not incorporated at detectable levels in glucose-containing polysaccharides or glycoconjugates. In contrast, carbon atoms from starch, a polymer of glucose, were incorporated into rhamnose in each of the three species examined. When grown in a serum-free medium supplemented with starch, M.arthritidis synthesized higher levels of rhamnose, with a shift in the relative amounts of the d and l configurations. Our findings suggest the presence of a novel pathway for rhamnose synthesis that is widespread in the genus Mycoplasma. © 2013 John Wiley & Sons Ltd.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Jordan DS; Daubenspeck JM; Dybvig K
  • Start Page

  • 918
  • End Page

  • 928
  • Volume

  • 89
  • Issue

  • 5