Single-cell isolation by a modular single-cell pipette for RNA-sequencing.

Academic Article

Abstract

  • Single-cell transcriptome sequencing highly requires a convenient and reliable method to rapidly isolate a live cell into a specific container such as a PCR tube. Here, we report a modular single-cell pipette (mSCP) consisting of three modular components, a SCP-Tip, an air-displacement pipette (ADP), and ADP-Tips, that can be easily assembled, disassembled, and reassembled. By assembling the SCP-Tip containing a hydrodynamic trap, the mSCP can isolate single cells from 5-10 cells per μL of cell suspension. The mSCP is compatible with microscopic identification of captured single cells to finally achieve 100% single-cell isolation efficiency. The isolated live single cells are in submicroliter volumes and well suitable for single-cell PCR analysis and RNA-sequencing. The mSCP possesses merits of convenience, rapidness, and high efficiency, making it a powerful tool to isolate single cells for transcriptome analysis.
  • Keywords

  • Animals, Cell Line, Tumor, Cell Separation, Mice, NIH 3T3 Cells, Sequence Analysis, RNA, Single-Cell Analysis
  • Digital Object Identifier (doi)

    Author List

  • Zhang K; Gao M; Chong Z; Li Y; Han X; Chen R; Qin L
  • Start Page

  • 4742
  • End Page

  • 4748
  • Volume

  • 16
  • Issue

  • 24