Background: Seat belt use has consistently been shown to reduce motor vehicle collision (MVC)-related morbidity and mortality. The goal of this study is to determine whether seat belt use is associated with fewer lost workdays among occupants involved in MVCs. Methods: The 1995 to 2000 National Automotive Sampling System (NASS) data files were used. The NASS is a national probability sample of passenger vehicles involved in police-reported tow-away MVCs. Occupants' lost workdays, which are routinely collected as part of an NASS investigation, were compared according to seat belt use. Results: During 1995 to 2000 in the United States, surviving occupants involved in MVCs lost a total of 42.1 million workdays (approximately 7.0 million lost workdays per year; 2.4 lost workdays per person). The overall difference in lost workdays between the belted and unbelted occupants was 1.59 days (1.99 days vs. 3.58 days, respectively; p < 0.001). After adjusting for potentially confounding factors, belted occupants had 1.52 fewer lost workdays compared with unbelted occupants (p < 0.001). This translates to an estimated 7.3 million lost workdays and an associated $566 million in lost wages and $1.25 billion in workplace costs attributable to lack of seat belt use in the United States during 1995 to 2000. Conclusion: Lost workdays attributable to MVCs in the United States have sizable financial implications. Furthermore, seat belt use significantly reduces lost time at work and is associated with a significant cost savings. The national impact of unbelted driving on work productivity is dramatic, and further efforts to promote appropriate seat belt use should continue as part of the national safety agenda.