Generation of Reactive Oxygen Species Mediated by 1-Hydroxyphenazine, a Virulence Factor of Pseudomonas aeruginosa

Academic Article

Abstract

  • © 2015 American Chemical Society. 1-Hydroxyphenazine (1-HP) is a virulence factor produced by Pseudomonas aeruginosa. In this study, supercoiled plasmid DNA was employed as an analytical tool for the detection of ROS generation mediated by 1-HP. These assays provided evidence that 1-HP, in conjunction with NADPH alone or NADPH and the enzyme NADPH:cytochrome P450 reductase, mediated the production of superoxide radical under physiological conditions. Experiments with murine macrophage RAW264.7 cells and profluorescent ROS probes dichlorodihydrofluorescein or dihydroethidine provided preliminary evidence that 1-HP mediates the generation of intracellular oxidants. Generation of reactive oxygen species may contribute to the virulence properties of 1-HP in P. aeruginosa infections.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Sinha S; Shen X; Gallazzi F; Li Q; Zmijewski JW; Lancaster JR; Gates KS
  • Start Page

  • 175
  • End Page

  • 181
  • Volume

  • 28
  • Issue

  • 2