15N-NMR of repeat peptides of tropoelastin. The tetrapeptide.

Academic Article

Abstract

  • The 15N-NMR data obtained in chloroform and methanol are reported for Boc-L-Val1-L-Pro2-Gly3-Gly4-OMe, a repeat tetrapeptide sequence of tropoelastin. Deuterium substitution for labile peptide protons was carried out to delineate solvent-exposed and solvent-shielded peptide nitrogens. Solvent perturbation of the peptide nitrogen resonances together with the hydrogen-deuterium substitution reaffirms the previously derived conformation of the molecule in chloroform which contains a beta-turn (type II) and a 14-membered hydrogen bond to form a cross-beta structure.
  • Published In

    Keywords

  • Chloroform, Elastin, Magnetic Resonance Spectroscopy, Methanol, Molecular Conformation, Nitrogen Isotopes, Oligopeptides, Tropoelastin
  • Digital Object Identifier (doi)

    Author List

  • Khaled MA; Okamoto K; Urry DW
  • Start Page

  • 229
  • End Page

  • 233
  • Volume

  • 623
  • Issue

  • 1