Coupling endoplasmic reticulum stress to cell death program in isolated human pancreatic islets: Effects of gene transfer of Bcl-2

Academic Article

Abstract

  • A variety of toxic insults can result in endoplasmic reticulum (ER)-stress that ultimately leads to apoptosis. β-cells have a highly developed ER due to a great commitment to insulin production. The present study was carried out to determine the role of ER-stress in isolated human pancreatic islet apoptosis, and the potential protective effects of Bcl-2. Isolated human islets were infected with an adenoviral vector encoding Bcl-2 and then exposed to brefeldin-A, tunicamycin, A23187 and pro-inflammatory cytokines. Activation of caspase-12 was analyzed by means of Western blots. Apoptosis was evaluated using a commercial quantitative assay. ER-stress-inducers promoted caspase-12 activation and apoptosis, effect reversed by overexpression of Bcl-2. Co-localization of caspase-12 and Bcl-2 in the microsomal islet fractions were demonstrated by means of Western blots. We can conclude that the current studies highlight the importance of Bcl-2 as an anti-apoptotic protein, and shed new light on the mechanisms underlying its cytoprotective effects on pancreatic islets.
  • Digital Object Identifier (doi)

    Author List

  • Contreras JL; Smyth CA; Bilbao G; Eckstein C; Young CJ; Thompson JA; Curiel DT; Eckhoff DE
  • Start Page

  • 537
  • End Page

  • 542
  • Volume

  • 16
  • Issue

  • 7