Investigating the pharmacokinetics and biological distribution of silver-loaded polyphosphoester-based nanoparticles using (111) Ag as a radiotracer.

Academic Article

Abstract

  • Purified (111) Ag was used as a radiotracer to investigate silver loading and release, pharmacokinetics, and biodistribution of polyphosphoester-based degradable shell crosslinked knedel-like (SCK) nanoparticles as a comparison to the previously reported small molecule, N-heterocyclic silver carbene complex analog (SCC1) for the delivery of therapeutic silver ions in mouse models. Biodistribution studies were conducted by aerosol administration of (111) Ag acetate, [(111) Ag]SCC1, and [(111) Ag]SCK doses directly into the lungs of C57BL/6 mice. Nebulization of the (111) Ag antimicrobials resulted in an average uptake of 1.07 ± 0.12% of the total aerosolized dose given per mouse. The average dose taken into the lungs of mice was estimated to be 2.6 ± 0.3% of the dose inhaled per mouse for [(111) Ag]SCC1 and twice as much dose was observed for the [(111) Ag]SCKs (5.0 ± 0.3% and 5.9 ± 0.8% for [(111) Ag]aSCK and [(111) Ag]zSCK, respectively) at 1 h post administration (p.a.). [(111) Ag]SCKs also exhibited higher dose retention in the lungs; 62-68% for [(111) Ag]SCKs and 43% for [(111) Ag]SCC1 of the initial 1 h dose were observed in the lungs at 24 h p.a.. This study demonstrates the utility of (111) Ag as a useful tool for monitoring the pharmacokinetics of silver-loaded antimicrobials in vivo.
  • Authors

    Keywords

  • aerosols, autoradiography, biodistribution, nanoparticles, nose-only inhalation, pharmacokinetics, radiotracer, silver antimicrobials, Administration, Inhalation, Animals, Anti-Infective Agents, Metal Nanoparticles, Mice, Organophosphorus Compounds, Silver, Tissue Distribution
  • Digital Object Identifier (doi)

    Authorlist

  • Aweda TA; Zhang S; Mupanomunda C; Burkemper J; Heo GS; Bandara N; Lin M; Cutler CS; Cannon CL; Youngs WJ
  • Start Page

  • 234
  • End Page

  • 241
  • Volume

  • 58
  • Issue

  • 6