Cyclotron Production of High-Specific Activity 55Co and In Vivo Evaluation of the Stability of 55Co Metal-Chelate-Peptide Complexes.

Academic Article

Abstract

  • This work describes the production of high-specific activity 55Co and the evaluation of the stability of 55Co-metal-chelate-peptide complexes in vivo. 55Co was produced via the 58Ni(p,α)55Co reaction and purified using anion exchange chromatography with an average recovery of 92% and an average specific activity of 1.96 GBq/μmol. 55Co-DO3A and 55Co-NO2A peptide complexes were radiolabeled at 3.7 MBq/μg and injected into HCT-116 tumor xenografted mice. Positron emission tomography (PET) and biodistribution studies were performed at 24 and 48 hours postinjection and compared to those of 55CoCl2. Both 55Co-metal-chelate complexes demonstrated good in vivo stability by reducing the radiotracers' uptake in the liver by sixfold at 24 hours with ~ 1% ID/g and at 48 hours with ~ 0.5% ID/g and reducing uptake in the heart by fourfold at 24 hours with ~ 0.7% ID/g and sevenfold at 48 hours with ~ 0.35% ID/g. These results support the use of 55Co as a promising new radiotracer for PET imaging of cancer and other diseases.
  • Published In

  • Molecular Imaging  Journal
  • Keywords

  • Animals, Chelating Agents, Colorectal Neoplasms, Contrast Media, Coordination Complexes, Cyclotrons, Female, HCT116 Cells, Humans, Mice, Mice, Nude, Peptides, Positron-Emission Tomography
  • Digital Object Identifier (doi)

    Pubmed Id

  • 25866336
  • Author List

  • Mastren T; Marquez BV; Sultan DE; Bollinger E; Eisenbeis P; Voller T; Lapi SE
  • Start Page

  • 526
  • End Page

  • 533
  • Volume

  • 14
  • Issue

  • 10