Properties of ATPase of gastric mucosa V. Preparation of membranes and mitochondria by zonal centrifugation

Academic Article

Abstract

  • Using zonal sucrose density gradient centrifugation methods to fractionate the subcellular components in gastric mucosal homogenates have been developed. Methods are described which give high yield or rapidity in fractionation. A procedure is described which enables large-scale preparations of smooth walled vesicular membranes containing HCO3--ATPase activity free from mitochondrial contamination as assessed by electron microscopic morphology and undetectable succinic dehydrogenase or monoamine oxidase activity. A method to purify gastric mitochondria is also described. © 1973.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Spenney JG; Strych A; Price AH; Helander HF; Sachs G
  • Start Page

  • 545
  • End Page

  • 564
  • Volume

  • 311
  • Issue

  • 4