Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts.

Academic Article

Abstract

  • Cultured cell lines and xenografts derived from 7 human medulloblastomas were evaluated for amplification of the c-myc, N-myc, epidermal growth factor receptor, and gli genes by Southern blot analysis. Karyotypes of the original biopsies and early passaged cells demonstrated double minute chromosomes in 4 of the 7 cases. All 7 samples (3 cell lines and 4 xenografts) from the 4 tumors with double minute chromosomes contained amplification of the c-myc gene. Cell lines and xenografts derived from the 3 biopsies without double minute chromosomes failed to demonstrate amplification of the 4 genes which were tested, but a rearrangement of the c-myc gene occurred in 1 of the 3 tumors. These observations demonstrate that the c-myc gene is often amplified and/or rearranged in human medulloblastomas and suggest that amplification of this gene provides a growth advantage for medulloblastoma cells in vitro and in vivo.
  • Authors

    Published In

  • Cancer Research  Journal
  • Keywords

  • Animals, Cell Line, Cerebellar Neoplasms, Chromosome Banding, DNA, Neoplasm, Female, Gene Amplification, Humans, Karyotyping, Male, Medulloblastoma, Mice, Mice, Nude, Neoplasm Transplantation, Nucleic Acid Hybridization, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-myc, Proto-Oncogenes, Transplantation, Heterologous
  • Author List

  • Bigner SH; Friedman HS; Vogelstein B; Oakes WJ; Bigner DD
  • Start Page

  • 2347
  • End Page

  • 2350
  • Volume

  • 50
  • Issue

  • 8