Virulence attenuation of a UDP-galactose/N-acetylglucosamine β1,4 galactosyltransferase expressing Leishmania donovani promastigote

Academic Article

Abstract

  • Protozoan parasites of the genus Leishmania are the causative agent of leishmaniasis, a disease whose manifestations in humans range from mild cutaneous lesions to fatal visceral infections. Human visceral leishmaniasis is caused by Leishmania donovani. Long-term culture in vitro leads to the attenuation of the parasite. This loss of parasite virulence is associated with the expression of a developmentally regulated UDP-Galactose/N-acetylglucosamine β 1-4 galactosyltransferase and galactose terminal glycoconjugates as determined by their agglutination with the pea nut agglutinin (PNA). Thus, all promastigotes passaged for more than 11 times were 100% agglutinated with PNA, and represent a homogeneous population of avirulent parasites. Identical concentrations of PNA failed to agglutinate promastigotes passaged for ≥5 times. These PNA- promastigotes were virulent. Promastigotes passaged from 5 to 10 times showed a mixed population. The identity of populations defined by virulence and PNA agglutination was confirmed by isolating PNA + avirulent and PNA- virulent clones from the 7th passage promastigotes. Only the PNA+ clones triggered macrophage microbicidal activity. The PNA+ clones lacked lipophosphoglycan. Intravenous administration of [14C] galactose-labeled parasite in BALB/c mice resulted in rapid clearance of the parasite from blood with a concomitant accumulation in the liver. By enzymatic assay and RT-PCR we have shown the association of a UDP-Galactose/N-acetylglucosamine β1,4 galactosyltransferase with only the attenuated clones. By immunofluorescence we demonstrated that the enzyme is located in the Golgi apparatus. By western blot analysis and SDS-PAGE of the affinity-purified protein, we have been able to identify a 29 KDa galactose terminal protein from the avirulent clones. © 2007 Springer Science+Business Media, LLC.
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    Author List

  • Bhaumik SK; Singh M; Basu R; Bhaumik S; Roychoudhury K; Naskar K; Roy S; De T
  • Start Page

  • 459
  • End Page

  • 472
  • Volume

  • 25
  • Issue

  • 5