Expression profiling of human sulfotransferase and sulfatase gene superfamilies in epithelial tissues and cultured cells

Academic Article

Abstract

  • The bioavailability of drugs administered topically or orally depends on their metabolism by epithelial enzymes such as the cytosolic sulfotransferases (SULT). Reverse transcriptase-polymerase chain reaction (RT-PCR) methods were established to detect expression of 8 SULT genes and 4 arylsulfatase (ARS) genes in human tissues of epithelial origin and in cultures of normal and transformed (cancer) cells. The results indicate: (i) SULT 1A1, 1A3, ARSC, and ARSD genes are ubiquitously expressed; (ii) expression is frequently similar between cell lines and corresponding tissues; (iii) SULT gene expression in normal cultured cells is generally comparable to the expression in associated transformed (cancer) cell lines; (iv) SULT 1A1 promoter usage is mainly tissue specific; however, both promoters are frequently used in SULT 1A3 expression; and (v) the expression profile of SULT 1A1, 1A3, 1E1, and 2B1a/b suggests that one or more of these isoforms may be involved in the cutaneous sulfoconjugation of minoxidil and cholesterol. (C) 2000 Academic Press.
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    Digital Object Identifier (doi)

    Author List

  • Dooley TP; Haldeman-Cahill R; Joiner J; Wilborn TW
  • Start Page

  • 236
  • End Page

  • 245
  • Volume

  • 277
  • Issue

  • 1